Despite their denaturing properties, detergents are used to purify and study membrane proteins. Herein, we demonstrated a polymer-based detergent-free extraction of the membrane protein cytochrome-b5 along with E. coli lipids. Nuclear magnetic resonance experiments revealed the suitability of using nanodiscs for high-resolution studies and revealed the types of native lipids associated with the protein.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337974 | PMC |
| http://dx.doi.org/10.1039/d0cc01737j | DOI Listing |
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