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Multiplex one-step direct asymmetric PCR of blood and dual-labelled probe-mediated melting curve for genotyping of MTHFR and MTRR polymorphisms.
RSC Adv
January 2025
Nanobiosensing and Microfluidic Point-of-Care Testing, Key Laboratory of Luzhou, Department of Clinical Laboratory, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University Luzhou Sichuan 646000 PR China
Accurate, rapid, and multiplex SNP analysis holds significant clinical value. However, the inevitable nucleic acid extraction, involving centrifugation, heating, and magnetic separation, is often time-consuming. In this study, direct blood PCR was combined with dual-labelled probe-mediated melting curves to identify SNPs corresponding to MTHFR (C677T, rs#1801133 and A1298C, rs#1801131) and MTRR (A66G, rs#1801394) in a single tube.
View Article and Find Full Text PDFStrain-tunable optoelectronic and photocatalytic properties of 2D GaN/MoSiP heterobilayers: potential optoelectronic/photocatalytic materials.
Nanoscale
January 2025
School of Science, Jiangsu University of Science and Technology, Zhenjiang 212001, China.
Herein, we propose a new GaN/MoSiP van der Waals (vdWs) heterostructure constructed by vertically stacking GaN and MoSiP monolayers. Its electronic, optical, and photocatalytic properties are explored DFT++BSE calculations. The calculated binding energy and phonon spectrum demonstrated the material's high stabilities.
View Article and Find Full Text PDFInference of Correlations Among Testlet Effects: A Latent Variable Selection Method.
Appl Psychol Meas
December 2024
Collaborative Innovation Center of Assessment Toward Basic Education Quality, Beijing Normal University, Beijing, China.
In psychological and educational measurement, a testlet-based test is a common and popular format, especially in some large-scale assessments. In modeling testlet effects, a standard bifactor model, as a common strategy, assumes different testlet effects and the main effect to be fully independently distributed. However, it is difficult to establish perfectly independent clusters as this assumption.
View Article and Find Full Text PDFPRESIUS, Predictive value of sonographic functional urethral length and urethral closure angle for the diagnosis of stress urinary incontinence.
Fr J Urol
December 2024
Centres médicaux-chirurgicaux Ambroise Paré, Hartmann, Pierre Cherest, 26, boulevard Victor-Hugo, 92200 Neuilly-sur-Seine, France. Electronic address:
Introduction: Numerous anatomical theories have been developed to explain women stress urinary incontinence (SUI) and improve its management. The transperineal ultrasound is an efficient and non-invasive exam that perfectly studies the bladder neck movement and the urethral anatomy. The measurement of the static portion of the distal urethral length, considered as the functional urethral length (FUL), and of the posterior urethral closure angle (PUCA) have not been studied before and could be of interest.
View Article and Find Full Text PDFDual-Wavelength On-Chip Integrated Metalens for Epi-Fluorescence Single-Molecule Sensing.
Sensors (Basel)
December 2024
Moscow Center for Advanced Studies, Kulakova str. 20, 123592 Moscow, Russia.
Single-molecule fluorescence spectroscopy offers unique capabilities for the low-concentration sensing and probing of molecular dynmics. However, employing such a methodology for versatile sensing and diagnostics under point-of-care demands device miniaturization to lab-on-a-chip size. In this study, we numerically design metalenses with high numerical aperture (NA = 1.
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