Preventative Approach to Microbial Control of by Soil Application of and .

Management of the Mediterranean flat-headed root-borer, Capnodis tenebrionis, is critical due to the larvae's root localization. Neonate larvae can be exposed to natural enemies before penetrating the roots. Application of strain Mb7 and strain GHA formulations on rice granules was investigated for their efficacy against larvae. Mb7 application, evaluated on apricot twigs, significantly and dose-dependently reduced colonization rates of neonates, with highest mortality at 10 conidia/g soil. Neonate susceptibility to Mb7 and GHA was evaluated on potted rootstocks (GF677 almond × peach, 2729 plum) planted in entomopathogenic fungi (EPF)-premixed soil (1.3-1.6 × 10 conidia/cm soil) or in EPF-free soil surface-treated with 5 g Mb7 fungal granules (1.25 × 10 conidia). Larval colonization rates were reduced 7.4-fold in 2729 by both fungi; only Mb7 completely prevented colonization of GF677 by larvae. Larvae inside plant galleries exhibited mycosis with EPF-treated soils and both fungi proliferated on larval frass. Mb7 conidia germinated in the rhizosphere of GF677, and conidia of both fungi remained viable throughout the trial. baiting technique was used on EPF-treated soil to evaluate EPF infectivity over time; Mb7 and GHA persisted 180 and 90 days post inoculation, respectively. The formulation (fungus-covered rice grains), delivery method (mixing with soil) and persistence (3-6 months) of Mb7 and GHA are feasible for potential field application to control .