Streptomyces is the most frequently described genus of Actinomycetes, a producer of biologically active secondary metabolites. Indeed, the Streptomyces species produces about 70% of antibiotics and 60% of antifungal molecules used in agriculture. Our study was carried out with the goal of isolating and identifying antimicrobial secondary metabolites from V16R3Y1 isolated from the date palm rhizosphere (southern Tunisia). This strain presented a broad range of antifungal activity against , , , , , , and and antibacterial activity against human pathogenic bacteria, including , , , , and . The purification procedure entailed ethyl acetate extract, silica gel column, and thin layer chromatography. Based on 1H NMR metabolomic procedure application, also supported by the GC-MS analysis, cyclic dipeptide (l-Leucyl-l-Proline) was identified as the major compound in the bioactive fraction. In order to confirm the identity of the active compound and to have a large quantity thereof, a chemical synthesis of the cyclic dipeptide was performed. The synthetic compound was obtained with a very good yield (50%) and presented almost the same effect compared to the extracted fraction. This study indicates for the first time that V16R3Y1 exhibits a broad spectrum of antimicrobial activities, produced cyclic dipeptide (l-Leucyl-l-Proline) and might have potential use as a natural agent for pharmaceutical and agri-food applications.
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http://dx.doi.org/10.3390/antibiotics9050270 | DOI Listing |
Mar Drugs
December 2024
CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Five new metabolites, including three cyclic dipeptide derivatives (-) and two new polyketides (-), together with nine known ones (- and -), were isolated from the mangrove-sediments-derived fungus sp. SCSIO 41431. Their structures were determined using detailed NMR, MS spectroscopic analyses, and quantum chemical calculations.
View Article and Find Full Text PDFJ Environ Sci Health B
December 2024
Department of Chemistry, B.M.S. College of Engineering, Bengaluru, Karnataka, India.
In this work, we fabricated the Fmoc-Pro-Phe-OMe modified carbon paste electrode (FPPO/MCPE) and used it for electrochemical detection of CP and FZ in a 0.1 M phosphate buffer solution (pH = 7). We characterized the Fmoc-Pro-Phe-OMe and applied it for the electrochemical detection of CP and FZ.
View Article and Find Full Text PDFChemphyschem
December 2024
Indian Institute of Technology Guwahati, Chemistry, Guwahati, 781039, Guwahati, INDIA.
A new class of cyclic dipeptide-isatin hybrids was synthesized using 2,5-diketopiperazine and various isatin derivatives bearing flexible chains and extended aromatic rings in a double Knoevenagel condensation process. These new series demonstrate high solubility and good thermal stability with decomposition temperatures exceeding 370 °C, especially the molecular design with efficient space-filling (CI5 and CI6) stabilized columnar liquid crystalline phase. The hole and electron carrier mobility of the CI5 compound, measured using the space charge limited current (SCLC) technique, were found to be 9.
View Article and Find Full Text PDFBiofilm
December 2024
Department of Life Sciences, School of Natural Sciences, Shiv Nadar Institution of Eminence, Delhi NCR, Greater Noida, 201314, India.
Microorganisms
November 2024
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Microorganism and Enzyme Research Center of Engineering Technology, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning 530004, China.
Microorganisms from poorly explored environments are promising sources for the development of novel drugs. In our continuous efforts to screen for mangrove actinomycetes that produce metabolites with potential pharmaceutical applications, sp. Y009 was isolated from mangrove sediments in Guangxi, China.
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