Cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC) and plastid glyceraldehyde-3-phosphate dehydrogenase (GAPCp) are key enzymes in glycolysis. Besides their catalytic function, GAPC/GAPCp participates in the regulation of plant stress response and growth and development. However, the involvement of GAPC/GAPCp in the regulation of fruit ripening is unclear. In this study, and 1 in strawberries were isolated and analyzed. and 1 transcripts showed high transcript levels in the fruit. Transient overexpression of and 1 delayed fruit ripening, whereas RNA interference promoted fruit ripening and affected fruit anthocyanins and sucrose levels. Change in the expression patterns of and 1 also influenced the expression of several glycolysis-related and ripening-related genes such as , , , , , , , , , and , and HO level and reduced glutathione (GSH)/glutathione disulfide (GSSG) redox potential. Meanwhile, metabolomics experiments showed that transient overexpression of 1 resulted in a decrease in anthocyanins, flavonoids, organic acid, amino acids, and their derivatives. In addition, abscisic acid (ABA) and sucrose treatment induced the production of large amounts of HO and inhibited the expression of /1 in strawberry fruit. These results revealed that /1 is a negative regulator of ABA and sucrose mediated fruit ripening which can be regulated by oxidative stress.
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| http://dx.doi.org/10.3390/genes11050580 | DOI Listing |
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