Objectives: To describe a method for the determination of urea in blood serum using urease enzyme and 3-MPA-capped CdTe quantum dots.
Materials And Methods: The method is based on the increase in pH of the solution as a result of the reaction between urea and urease, which causes an increase in the phosphorescence signal of MPA-CdTe quantum dots in the pH range of 2.5-5.0. Under the optimum conditions, the linear range of urea was 0.016-0.16 mM (1-10 mg/L) and the limit of detection based on 3 s/b was calculated as 0.003 mM (0.17 mg/L). The relative standard deviation was calculated as 3.4% at 4 mg/L urea concentration (n=7).
Results: The method was applied to human serum samples. The same samples were analyzed by an independent laboratory and the results were not statistically different, at 95% confidence level (F test).
Conclusion: The proposed method does not need sample pretreatment, is simple, selective, and cost-effective for the determination of urea in serum samples.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227896 | PMC |
| http://dx.doi.org/10.4274/tjps.02986 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Fluorescent CdTe/ZnS Core/Shell Quantum Dots for Sensitive Metabolite Detection in Real Samples.
J Fluoresc
January 2025
Scientific and Technological Researches Application and Research Center, Duzce University, Duzce, Türkiye.
This study highlights the aqueous synthesis of CdTe/ZnS core/shell quantum dots (QDs) and their application as fluorescence sensors for detecting critical metabolites, including folic acid, glucose, and vitamin C, in real biological samples. The synthesized QDs exhibit excellent quantum efficiency, stability, and biocompatibility, enhanced by mercaptopropionic acid (MPA) ligands, enabling eco-friendly and accurate sensing. Detection limits of 0.
View Article and Find Full Text PDFInhibitory effects of cadmium and hydrophilic cadmium telluride quantum dots on the white rot fungus .
Heliyon
January 2025
Department of Microbiology (Biocenter 1, Viikinkaari 9), Faculty of Agriculture and Forestry, University of Helsinki, Finland.
The white rot fungus was investigated for its ability to decolorize the reactive textile dye Reactive Black 5 (RB5) that was co-exposed to CdCl and quantum dots (QDs) consisting of a CdTe core capped with two different hydrophilic organic ligands (NAC and MPA). Without co-exposure, completely decolorizes RB5 within 9 days. The highest inhibitory effect was found for soluble CdCl with an EC of 583 μg l, followed by MPA-QDs (10,628 μg l) and NAC-QDs (17,575 μg l).
View Article and Find Full Text PDFReconstruction of local three-dimensional temperature field of tumor cells with low-toxic nanoscale quantum-dot thermometer and cepstrum spatial localization algorithm.
Biomed Phys Eng Express
January 2025
State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, People's Republic of China.
The optimal method for three-dimensional thermal imaging within cells involves collecting intracellular temperature responses while simultaneously obtaining corresponding 3D positional information. Current temperature measurement techniques based on the photothermal properties of quantum dots face several limitations, including high cytotoxicity and low fluorescence quantum yields. These issues affect the normal metabolic processes of tumor cells.
View Article and Find Full Text PDFA molecularly imprinted ratio fluorescence sensor based on metal-enhanced fluorescence of core-shell structure CaF-silver nanoparticle for visual detection of dicamba.
Anal Bioanal Chem
January 2025
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
Although fluorescence analysis methods are widely used in pesticide residue detection, improving their sensitivity and selectivity remains a challenge. This paper presents a novel ratio fluorescence sensor based on the molecular imprinting polymers (MIPs) and metal-enhanced fluorescence for visual detection of dicamba (DIC). Calcium fluoride (CaF) quantum dots (QDs) were immobilized on the surface of Ag@MIPs, resulting in a blue fluorescence response signal (Ag@MIPs-CaF).
View Article and Find Full Text PDFLow-Toxicity and High-Stability Fluorescence Sensor for the Selective, Rapid, and Visual Detection Tetracycline in Food Samples.
Molecules
December 2024
State Key Laboratory of Molecular Engineering of Polymers, Laboratory of Advanced Materials, Shanghai Key Lab of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200438, China.
With the development and improvement of analysis and detection systems, low-toxicity and harmless detection systems have received much attention, especially in the field of food detection. In this paper, a low-toxicity dual-emission molecularly imprinted fluorescence sensor (CdTe QDs@SiO/N-CDs@MIPs) was successfully designed for highly selective recognition and visual detection of tetracycline (TC) in food samples. Specifically, the non-toxic blue-emission N-doped carbon dots (N-CDs) with high luminous performance acted as the response signals to contact TC via the covalent bond between amino and carboxyl groups.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!