AI Article Synopsis
- * Researchers developed a fluorescent assembly using a dye (BOD-Ad) that can detect E. coli through a chemical reaction involving a protein (BSA) and copper binding, which changes its fluorescence properties.
- * This new method can identify live E. coli in the environment with a detection limit of 6.3 CFU/mL, making it a promising tool for hygienic assessments.
Article Abstract
Escherichia coli (E. coli) is abundantly present in nature. It is generally harmless to humans but some strains have been deemed very dangerous. Therefore, as an indicator of hygienic testing, the detection of E. coli is essential. In this work, a fluorescent assembly was synthesized and characterized by spectroscopic methods. It was found that the amantadine (Ad) conjugated dye (BOD-Ad) intercalated into Cmyc G4 (aptamer) forming a non-emission assembly (BOD-Ad-Cmyc), which could be lighted-up by BSA due to the formation of fluorescence nanoparticle BOD-Ad-Cmyc@BSA. Further, BOD-Ad-Cmyc@BSA can selectively bind Cu forming non-emission species BOD-Ad-Cmyc@BSA-Cu. E. coli can turn-on the emission of BOD-Ad-Cmyc@BSA-Cu system due to the copper accumulation or reduction by E. coli. Therefore, a fluorescence method for the determination of E. coli was built. The detection limit of BOD-Ad-Cmyc@BSA-Cu of E. coli is 6.3 CFU/mL. Thus, this BOD-Ad-Cmyc@BSA-Cu fluorescent assembly can be used for the detection of live E. coli in the environment.
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| http://dx.doi.org/10.1016/j.saa.2020.118483 | DOI Listing |
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