Vitellogenins and choriogenins are biomarkers for monitoring Oryzias curvinotus juveniles exposed to 17 β - estradiol.

The effect of estrogens on Oryzias curvinotus juveniles were investigated by sequencing the transcriptome of O. curvinotus juveniles exposed to 17 β - estradiol for 24 h. A total of 69,071,524 and 71,210,528 raw reads were obtained for the control group (NC) and 17 β - estradiol exposure group (E2), respectively. After de novo assembly, total 133,210 unigenes were identified, and 85,837 unigenes (64.44% of 133,210) were annotated. Analysis of the transcriptome showed that exposure to 2 μg/L 17 β - estradiol led to the up-regulation of 19 genes and down-regulation of 18 genes. The eef1b and rps4x was most suitable as controls for quantitative real-time PCR (qPCR) using Reffinder. Different expression genes enrichment analysis found that exposed to 2 μg/L 17 β - estradiol affected various physiological processes, including spliceosome, phototransduction, amino sugar and nuclear sugar metabolism, hypotaurine metabolism, and renin-angiotensin system, etc. Exposing O. curvinotus juveniles to increasing concentrations of 17 β - estradiol (2 ng/L, 20 ng/L, 200 ng/L and 2 μg/L) led to significant up-regulation of vitellogenins (vtgs) and choriogenins (chgs) mRNA expression. The present study is the first high-throughput transcriptome sequencing of O. curvinotus juveniles, which will be useful for future functional analysis of genes related to environmental estrogen exposed, and development of biomarkers.