AI Article Synopsis
- The study created induced pluripotent stem (iPS) cell lines using fibroblasts from the dura mater of four deceased individuals who had low genetic risk for psychiatric disorders like schizophrenia and bipolar disorder.
- The fibroblasts were reprogrammed into iPS cells using specific genetic factors delivered through episomal vectors.
- All iPS cell lines maintained the same genetic profile as the original brain tissues, expressed markers indicating pluripotency, and demonstrated the ability to differentiate into all three embryonic germ layers.
Article Abstract
In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10515715 | PMC |
| http://dx.doi.org/10.1016/j.scr.2020.101806 | DOI Listing |
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