Recent Progress on the Electrochemical Biosensing of O157:H7: Material and Methods Overview.

O157:H7 ( O157:H7) is a pathogenic strain of which has issued as a public health threat because of fatal contamination of food and water. Therefore, accurate detection of pathogenic is important in environmental and food quality monitoring. In spite of their advantages and high acceptance, culture-based methods, enzyme-linked immunosorbent assays (ELISAs), polymerase chain reaction (PCR), flow cytometry, ATP bioluminescence, and solid-phase cytometry have various drawbacks, including being time-consuming, requiring trained technicians and/or specific equipment, and producing biological waste. Therefore, there is necessity for affordable, rapid, and simple approaches. Electrochemical biosensors have shown great promise for rapid food- and water-borne pathogen detection. Over the last decade, various attempts have been made to develop techniques for the rapid quantification of O157:H7. This review covers the importance of O157:H7 and recent progress (from 2015 to 2020) in the development of the sensitivity and selectivity of electrochemical sensors developed for O157:H7 using different nanomaterials, labels, and electrochemical transducers.