Genotoxicity and oxidative damage in zebrafish (Danio rerio) after exposure to effluent from ethyl alcohol industry.

In this research, the toxicological effect of untreated wastewater from of ethyl alcohol industry was evaluated on the zebrafish (Danio rerio) under experimental conditions. Fish were treated with zero, half, one and two percent of sewage effluent for 21 days. Toxic effects were monitored in liver by determining biochemical indicators, oxidative stress biomarkers, and the expression of genes involved in the detoxification. Results showed that Sod1, Gstp-1a, Gpx1a gene expressions were significantly increased in the hepatocytes after 21 days at 2.0% sewage exposure. Sewage exposure also significantly increased Gsr, Ces2 and Cyp1a, Mt1 and Mt2 gene expression in the hepatocytes of zebrafish as compared to the reference group (P < 0.01). Total cellular antioxidants, malondialdehyde (MDA) levels, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) activities in fish exposed to 1 and 2% of sewage were significantly higher than the control group (P < 0.01), whereas alanine aminotransferase (ALT) was only increased in fish exposed to 2% sewage (P < 0.01). A significant decrease in gamma-glutamyl-transferase (GGT) activity in fish exposed to 2% effluent was found (P < 0.01). Catalase (CAT) activity was increased in zebrafish exposed to all concentrations of effluent. The transcriptional analysis of the detoxification-related genes and the changes in the biochemical indicators evidenced that drainage of sewage effluents from the ethyl alcohol company is a serious threat to the health of aquatic animals in the Khorram-Rood River. These results will contribute to further study on the impact of sewage effluents of the alcohol industry on aquatic organisms.