AI Article Synopsis
- * ASPO demonstrated significant anti-hepatoma effects against SMMC-7721 cells, with an IC value of 15.96 μg/mL and a tumor inhibition rate of 54.14% at a dosage of 50 mg/kg.
- * Protein analysis revealed that ASPO induces apoptosis in cancer cells by manipulating key proteins like Bcl-2 and Bax, and engages specific pathways such as Fas/FasL and Bcl-2/Bax pathways.
Article Abstract
In this study, an optimal method used to extract pericarp oil (ASPO) was established according to the response surface model. The yield of ASPO was 1.45%. 8 fatty acids were identified from ASPO by GC-MS. Among them, ()-9-Octadecenoic acid was abundant and accounted for 49.65%. The anti-hepatoma activities of ASPO were investigated against SMMC-7721 cell line in and H cell line . Proteins associated with apoptosis in tumour tissue were quantified by western blot assay. The result revealed that ASPO had significant anti-hepatoma activities with IC value of 15.96 μg/mL and tumour inhibition rate of 54.14% at 50 mg/kg dose Protein analysis showed that ASPO activated apoptosis by down-regulating Bcl-2, up-regulating Bax, cleaving caspase 9, cleaving caspase 8 and cleaving caspase 3 proteins. The possible mechanisms of apoptosis induced by ASPO were related to Fas/FasL/caspase-8/caspase-3 and Bcl-2/bax/caspase-9/caspase-3 pathways.
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| http://dx.doi.org/10.1080/14786419.2020.1765346 | DOI Listing |
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