is one of the major stomach microbiome components, promoting development of inflammation and gastric cancer in humans. has a unique ability to transform into a coccoidal form which is difficult to detect by many diagnostic methods, such as urease activity detection, and even histopathological examination. Here we present a comparison of three methods for identification: histological assessment (with eosin, hematoxylin, and Giemsa staining), polymerase chain reaction (PCR) detection of urease (ureA specific primers), and detection by 16S rRNA gene sequencing. The study employed biopsies from the antral part of the stomach ( = 40). All samples were assessed histologically which revealed in eight patients. Bacterial DNA isolated from the bioptates was used as a template for PCR reaction and 16S rRNA gene sequencing that revealed in 13 and in 20 patients, respectively. Thus, 16S rRNA gene sequencing was the most sensitive method for detection of in stomach biopsy samples.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229771 | PMC |
| http://dx.doi.org/10.7717/peerj.9099 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
sp. nov., an acidotolerant sulphate-reducing bacterium isolated from moderately acidic fen soil.
Int J Syst Evol Microbiol
January 2025
Department of Microorganisms, Leibniz Institute DSMZ German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
An obligately anaerobic, spore-forming sulphate-reducing bacterium, strain SB140, was isolated from a long-term continuous enrichment culture that was inoculated with peat soil from an acidic fen. Cells were immotile, slightly curved rods that stained Gram-negative. The optimum temperature for growth was 28 °C.
View Article and Find Full Text PDFsp. nov., isolated from tree bark ( Chev.) and its antioxidant activity.
Int J Syst Evol Microbiol
January 2025
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
A Gram-stain-positive, facultatively anaerobic, rod-shaped strain, designated SPB1-3, was isolated from tree bark. This strain exhibited heterofermentative production of dl-lactic acid from glucose. Optimal growth was observed at 25-40 °C, pH 4.
View Article and Find Full Text PDFgen. nov., sp. nov.: a novel species of a novel genus in the family , isolated from the sediment of a tidal flat located in Zhoushan, China.
Int J Syst Evol Microbiol
January 2025
College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
A Gram-stain-negative, aerobic and rod-shaped bacterium, designated as HZG-20, was isolated from a tidal flat in Zhoushan, Zhejiang Province, China. The 16S rRNA sequence similarities between strain HZG-20 and RR4-56, NNCM2, P31 and X9-2-2 were 98.9, 91.
View Article and Find Full Text PDFGenomic and phenotypic characterisation of Leeuwenhoekiella obamensis sp. nov., a novel marine bacterium isolated from the surface water of a Japanese fishing port.
Antonie Van Leeuwenhoek
January 2025
Department of Marine Science and Technology, Fukui Prefectural University, Obama, Fukui, 917-0003, Japan.
A novel aerobic marine bacterium, FRT2, isolated from surface water of a fishing port in Fukui, Japan, was characterised based on phylogenomic and phylogenetic analyses combined with classical phenotypic and chemotaxonomic characterisations. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FRT2 clustered with genus Leeuwenhoekiella. Closest relatives of FRT2 were Leeuwenhoekiella palythoae KMM 6264 and Leeuwenhoekiella nanhaiensis G18 with 16S rRNA gene sequence identities of 95.
View Article and Find Full Text PDFDistinct Ocular Surface Microbiome in Keratoconus Patients Correlate With Local Immune Dysregulation.
Invest Ophthalmol Vis Sci
January 2025
GROW Research Laboratory, Narayana Netralaya Foundation, Bangalore, India.
Purpose: Keratoconus (KC) is characterized by irregular astigmatism along with corneal stromal weakness and is associated with altered immune status. Tissue resident microbiomes are known to influence the immune status in other organs, but such a nexus has not been described in ocular conditions. Therefore, we examined the ocular surface microbiome of patients with KC and correlated it to the immune cell and tear molecular factor profiles.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!