Aim: To determine the effect of three different cavity disinfectants (2% chlorhexidine gluconate, 2.5% sodium hypochlorite, and 2% iodine solution) on microleakage in a seventh-generation dentin-bonding system.

Materials And Methods: Class V cavity was prepared on 50 extracted molars ( = 50). The respective experimental groups were treated with cavity disinfectants and Adper Easy One Bond. Preparations without cavity disinfectants worked as negative control and those with neither disinfectant nor dentin-bonding resin application worked as positive controls. After the cavity preparations were restored with resin composite (Filtek™ Z 350), the teeth were then subjected to dye leakage tests. Microleakage was assessed for both occlusal and gingival margins, using a stereomicroscope. Data were analyzed using (ANOVA; Kruskal-Wallis) test.

Results: No statistically significant differences were observed among 2% chlorhexidine gluconate, 2.5% sodium hypochlorite, and 2% iodine and also no statistically significant differences were observed between occlusal and gingival margins of groups.

Conclusion: (1) 2% chlorhexidine gluconate, 2.5% sodium hypochlorite, and 2% iodine produced significantly higher microleakage when used with seventh-generation dentin-bonding agent. (2) 2% chlorhexidine gluconate produced lesser microleakage in comparison with 2.5% sodium hypochlorite and 2% iodine. (3) The gingival margins exhibited greater microleakage than occlusal margins.

Clinical Significance: The application of cavity disinfectants on prepared tooth before the application of dentin-bonding agent could help to reduce the potential risk of residual caries and postoperative sensitivity.

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