As a crucial member of the Forkhead Box family, class O (FoxO) plays an essential role in growth, cell differentiation, metabolism, immunization, and apoptosis. Meanwhile, FoxO3 is the primary regulator and effective inhibitor of primordial follicle activation. In this study, seven foxo genes were identified in black rockfish (Sebastes schlegelii), including two foxo1 genes (foxo1a, foxo1b), two foxo3 genes (foxo3, foxo3l), one foxo4 gene, and two foxo6 genes (foxo6a, foxo6b). foxo3l was derived from teleost-specific whole-genome duplication events. Evaluation of tissue expression pattern revealed that foxo3l displayed sexually dimorphic expression with a high level in the ovary and spatial expression only in the cytoplasm of follicle cells and oocytes. When the ovaries were stimulated by estrogen and gonadotropin, foxo3l expression was remarkably reduced, and the effect of androgen was completely different. We considered that foxo3l lost its ability to inhibit follicular precocity because of mass ovulation by hormone stimulation, resulting in its decreased expression. Such evidence indicated that foxo3l is an important regulator of reproduction-related functions in black rockfish. This study provides new insights into foxo3l genes for further functional research in teleost.
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Article Synopsis
- The study focused on sperm storage in black rockfish and how the female reproductive system adapts to this, revealing that sperm are stored in specific locations during different reproductive stages.
- A total of 5012 proteins were identified, showing significant changes in expression at the pre-fertilization stage, with 140 proteins upregulated and 615 downregulated compared to pre-mating.
- The research indicated that specific signaling pathways related to vesicle sorting, hormone signaling, and immune response are crucial for oocyte development and sperm storage, providing useful insights for reproductive biology and aquaculture.
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