Background: American Foulbrood (AFB) is a devastating disease of honey bee () larvae caused by the spore-forming, Gram-positive bacterium . In most countries, the law requires mandatory reporting of AFB to the veterinary authority.
Aim And Methods: To speed up detection and genotyping of spores, we compared different culturing protocols on Columbia sheep blood agar and developed a new multiplex quantitative polymerase chain reaction to distinguish between the two relevant genotypes enterobacterial repetitive intergenic consensus (ERIC) I and ERIC II.
Results And Conclusion: As confirmed by reference strains and field isolates, the new identification and genotyping protocol halves the time of current workflows, lessens labor-intension, allows a higher throughput of samples for monitoring, and permits a faster intervention to prevent the spread of AFB.
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| http://dx.doi.org/10.4314/ovj.v10i1.9 | DOI Listing |
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