A magnetic multi-walled carbon nanotube preparative method for analyzing asymmetric carbon, phosphorus and sulfur atoms of chiral pesticide residues in Chinese herbals by chiral liquid chromatography-quadrupole/linear ion trap mass spectrometry determination.

An analytical method for the determination of asymmetric carbon, phosphorus and sulfur atoms in chiral pesticide residues by magnetic multi-walled carbon nanotube sample pretreatment combined with chiral ultra-performance liquid chromatography/quadrupole/linear ion trap mass spectrometry (UPLC-MS/Qtrap) was developed and applied to chiral pesticide residues analysis in Chinese herbals. Eleven different chiral pesticides were found, and 36.4% were positive in Chinese herbals. Three plants containing detectable pesticide residues were observed in Dendrobium nobile, Panax notoginseng flowers and honeysuckle, in the order of decreasing detected concentration. High detection frequencies of 26.1% for (R/S)-(±)-difenoconazole and 14.5% for (R/S)-(±)-metalaxyl and (R/S)-(±)-propiconazole were observed, the residual amount for (R/S)-(±)-difenoconazole, (R/S)-(±)-metalaxyl and (R/S)-(±)-propiconazole were 0.32 ~ 2.5 mg/kg, 0.022 ~ 0.23 mg/kg, 0.62 ~ 3.21 mg/kg respectively. The EF value of (R/S)-(±)-difenoconazole was 0.506 ± 0.046. The EF value of (R/S)-(±)-metalaxyl was lower than 0.5 in Dendrobium nobile, Panax notoginseng flowers, Panax notoginseng roots and hawthorn. The EF of (R/S)-(±)-propiconazole was not significantly enantioselective in honeysuckle and Panax notoginseng flowers. The enantioselectivity of various pesticide residues in different plants cannot be predicted from our existing knowledge and may closely depend on plant growth, environmental conditions or molecular structure.