Tea extract increases cell fusion via regulation of cell surface DC-STAMP.

Biochem Biophys Rep

Department of Life Sciences, Graduate School of Bioresources, Mie University, 1577, Kurimamachiya, Tsu, Mie, 514-8507, Japan.

Published: July 2020

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Article Abstract

Mononuclear osteoclast precursor cells fuse with each other to become mature multinucleated osteoclasts, which is regulated by dendritic cell-specific transmembrane protein (DC-STAMP). We evaluated the effects of tea extract and catechins on cell-cell fusion and DC-STAMP expression to elucidate their relationship with osteoclast development. When tea extract or epigallocatechin gallate (EGCg) was applied to RAW264.7 cells, multinucleated cells were increased significantly, while tartrate-resistant acid phosphatase (TRAP) activity was hardly upregulated. Flow cytometric analysis revealed that EGCg suppressed DC-STAMP expression on the cell surface, which is similar to osteoclast development. These observations suggest that TRAP activity is not activated even when suppression of both surface DC-STAMP expression and multinucleation occurs, which might be mediated by another pathway.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218152PMC
http://dx.doi.org/10.1016/j.bbrep.2020.100759DOI Listing

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