AI Article Synopsis
- Hydroxysafflor yellow A (HSYA), a key ingredient in Chinese medicine, exhibits various pharmacological effects, and its absorption and fluorescence properties were studied under different conditions.
- The fluorescence intensity of HSYA is significantly influenced by factors like acidity, temperature, viscosity, and surfactants, especially increasing with the presence of borax, which enhances it by 20 times.
- The developed borax-HSYA sensitization system is nontoxic to cells and animals, offering a reliable method for studying HSYA's behavior and metabolic processes through fluorescence imaging.
Article Abstract
As the main active ingredient in Chinese medicine safflower, hydroxysafflor yellow A (HSYA) has multiple pharmacological effects. In the work, the absorption and fluorescence spectra of HSYA under different environmental conditions (such as acidity, temperature, ions, viscosity, and surfactant) were investigated. The fluorescence intensity of HSYA varied greatly with acidity, temperature, viscosity, and surfactant, but was less affected by common cations and anions. Among various surfactants, we found that borax can significantly enhance the HSYA fluorescence intensity, and thus, a borax-HSYA sensitization system for HSYA fluorescence was established. In the optimized sensitization system, the fluorescence intensity of HSYA increased by 20 times and showed a good linearity with HSYA concentrations in the range of 0∼10 M with a detection limit of 8 nM. The borax-HSYA sensitization system is nontoxic to T24 cells and mice and can be used for the fluorescence imaging of HSYA in cells, thereby providing an effective method for analyzing HSYA and monitoring its metabolism in cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204355 | PMC |
| http://dx.doi.org/10.1155/2020/3027843 | DOI Listing |
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