The effect of sulfate on nitrite-denitrifying anaerobic methane oxidation (nitrite-DAMO) process.

Sulfate is generally found in natural water and wastewater. Nitrite-DAMO bacteria live in natural water or wastewater containing different sulfates. To determine the effect of sulfate on the nitrite-DAMO process, we conducted batch tests and continuous tests to investigate the performance and microbial structure of the nitrite-DAMO system at different sulfate concentrations. The results indicated that the nitrogen removal performance of the nitrite-DAMO system was initially promoted and then inhibited at 0-200 mg SO/L, and the denitrification rate was highest at 80 mg SO/L which was 1.26 mgN/(L·d). When stimulated by sulfate, protein stabilized nitrite-DAMO bacteria. The denitrification kinetics conformed to the Edward equation, and the initial inhibitory concentration of the nitrite-DAMO system was 189.70 mg SO/L. Changes in the proportion of unclassfied_c_ABY1 of the phylum Patescibacteria and norank_f_LD-RB-34 of the phylum Bacteroidetes were the main factors influencing how the nitrogen removal rate of the nitrite-DAMO system responded to sulfate.