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The SecA ATPase motor protein binds to Escherichia coli liposomes only as monomers. | LitMetric

The SecA ATPase motor protein binds to Escherichia coli liposomes only as monomers.

Biochim Biophys Acta Biomembr

Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, Irvine, CA 92697, United States of America. Electronic address:

Published: September 2020

AI Article Synopsis

Article Abstract

The essential SecA motor ATPase acts in concert with the SecYEG translocon to secrete proteins into the periplasmic space of Escherichia coli. In aqueous solutions, SecA exists largely as dimers, but the oligomeric state on membranes is less certain. Crystallographic studies have suggested several possible solution dimeric states, but its oligomeric state when bound to membranes directly or indirectly via the translocon is controversial. We have shown using disulfide crosslinking that the principal solution dimer, corresponding to a crystallographic dimer (PDB 1M6N), binds only weakly to large unilamellar vesicles (LUV) formed from E. coli lipids. We report here that other soluble crosslinked crystallographic dimers also bind weakly, if at all, to LUV. Furthermore, using a simple glutaraldehyde crosslinking scheme, we show that SecA is always monomeric when bound to LUV formed from E. coli lipids.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7316483PMC
http://dx.doi.org/10.1016/j.bbamem.2020.183358DOI Listing

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