Diversity Revealed by Targeted Amplicon Sequencing of Gene, Metabolic Comparisons and Antimicrobial Properties in an Undefined Mixed Starter Culture Used for Soft-Cheese Manufacture.

The undefined mixed starter culture (UMSC) is used in the manufacture of cheeses. Deciphering UMSC microbial diversity is important to optimize industrial processes. The UMSC was studied using culture-dependent and culture-independent based methods. MALDI-TOF MS enabled identification of species primarily from the genus. Comparisons of carbohydrate metabolism profiles allowed to discriminate five phenotypes of ( = 26/1616). The 16S sequences analysis (V1-V3, V3-V4 regions) clustered the UMSC microbial diversity into two operational taxonomic units (OTUs). These clustering results were improved with the DADA2 algorithm on the housekeeping sequences. Five variants were detected among the UMSC. The whole-genome sequencing of six isolates allowed for the identification of the subspecies using Illumina ( = 5) and Pacbio ( = 1) technologies. Kegg analysis confirmed the species-specific niche adaptations and highlighted a progressive gene pseudogenization. Then, agar spot tests and agar well diffusion assays were used to assess UMSC antimicrobial activities. Of note, isolate supernatants ( = 34/1616) were shown to inhibit the growth of ser. Typhimurium CIP 104115, CIP 104494, DSMZ 13661, CIP103015 and CIP 80.11. Collectively, these results provide insightful information about UMSC diversity and revealed a potential application as a bio-protective starter culture.