Estimating post-mortem interval is an important aspect in forensic investigations. The aim of this study was to investigate if time-lapse imaging can be used to improve estimates of post-mortem interval using Megyesi et al.'s [1] method for a human donor decomposing in an Australian environment. To achieve this, time-lapse images were taken every 30 min over a 6-month period. The Megyesi et al. [1] total body score (TBS) system was used to quantify the level of decomposition of the donor for each image. Linear regression was performed to determine if observing decomposition more than once a day leads to increased accuracy in predicting PMI (post-mortem interval). Decomposition initially progressed quickly and then plateaued at 1004 hours PMI, with a TBS of 24. Individual timestamps were created from the time-lapse images taken each day at 08:00 hrs, 11:00 hrs, 14:00 hrs, 15:00 hrs, and 17:00 hrs. All timestamps produced R values > 0.80, indicating that the Megyesi et al. [1] method accurately predicts PMI for this donor. The 08:00 hrs timestamp had the highest value R = 0.886, whilst the combined timestamp (which included the scores from all five images for each 24-hour period) R = 0.823 was the lowest. This study supports the validity of Megyesi et al.'s [1] TBS model to estimate PMI. Two other interesting findings were that the results suggest that scoring TBS multiple times per day does not improve estimates of PMI, however scoring TBS at daybreak produces more accurate results than scoring TBS later in the day. This may be an important consideration in forensic scenarios.
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http://dx.doi.org/10.1016/j.fsisyn.2019.08.003 | DOI Listing |
Ann Neurol
January 2025
Research Unit of Neurology, Neurophysiology and Neurobiology, Department of Medicine and Surgery, Università Campus Bio-Medico di Roma, Rome, Italy.
Objective: Despite diagnostic criteria refinements, Parkinson's disease (PD) clinical diagnosis still suffers from a not satisfying accuracy, with the post-mortem examination as the gold standard for diagnosis. Seminal clinicopathological series highlighted that a relevant number of patients alive-diagnosed with idiopathic PD have an alternative post-mortem diagnosis. We evaluated the diagnostic accuracy of PD comparing the in-vivo clinical diagnosis with the post-mortem diagnosis performed through the pathological examination in 2 groups.
View Article and Find Full Text PDFBiomedicines
January 2025
Faculty of Law and Criminology, Maastricht University, Minderbroedersberg 4-6, 6211 LK Maastricht, The Netherlands.
The excitability of skeletal muscle is a less-known post-mortem supravital phenomenon in human bodies, and it can be used to estimate the post-mortem interval. We conducted a field study in the Netherlands to investigate the applicability of muscle excitability (SMR) by mechanical stimulation for estimating the post-mortem interval in daily forensic practice. Knowledge concerning the post-mortem cell mechanisms accounting for the post-mortem excitability of skeletal muscle is lacking.
View Article and Find Full Text PDFSci Justice
January 2025
Laboratory of Criminalistics, Adam Mickiewicz University in Poznań, al. Niepodległości 53, Poznań 61-714, Poland; Centre for Advanced Technologies, Adam Mickiewicz University in Poznań, ul. Uniwersytetu Poznańskiego 10, Poznań 61-614, Poland.
While estimating postmortem interval (PMI) ambient temperature plays a pivotal role, so its reconstruction is crucial for forensic scientists. The recommended procedure is to correct temperatures from the nearest meteorological station based on measurements from the death scene; typically applying linear regression. Recently, there have been attempts to use different algorithms, that can improve that correction, for example GAM algorithm.
View Article and Find Full Text PDFFront Mol Biosci
January 2025
Department of Forensic Medicine, Medical University of Bialystok, Bialystok, Poland.
Introduction: Accurate post-mortem interval (PMI) estimation is essential in forensic investigations. Although various methods for PMI determination have been developed, only an approximate estimation is still achievable, and an accurate PMI indication is still challenging. Therefore, in this study, we employed gas chromatography-mass spectrometry (GC-MS)-based metabolomics to assess post-mortem changes in porcine blood samples collected with and without the addition of anticoagulant (EDTA).
View Article and Find Full Text PDFCommun Biol
January 2025
Cardio-Thoracic Translational Medicine (CTTM) Lab, Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.
Recent developments in mass spectrometry-based proteomics have established it as a robust tool for system-wide analyses essential for pathophysiological research. While post-mortem samples are a critical source for these studies, our understanding of how body decomposition influences the proteome remains limited. Here, we have revisited published data and conducted a clinically relevant time-course experiment in mice, revealing organ-specific proteome regulation after death, with only a fraction of these changes linked to protein autolysis.
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