AI Article Synopsis
- DNA methylation is a key epigenetic mechanism influencing gene expression, and profiling it is crucial for understanding health and disease.
- Traditional methods for profiling DNA methylation involve costly and time-consuming chemical modifications and sequencing.
- A new electric biosensor using a graphene field-effect transistor enables rapid, label-free detection of DNA methylation, successfully profiling distinct methylated forms associated with glioblastoma at very low concentrations.
Article Abstract
DNA methylation is one of the principal epigenetic mechanisms that control gene expression in humans, and its profiling provides critical information about health and disease. Current profiling methods require chemical modification of bases followed by sequencing, which is expensive and time-consuming. Here, we report a direct and rapid determination of DNA methylation using an electric biosensor. The device consists of a DNA-tweezer probe integrated on a graphene field-effect transistor for label-free, highly sensitive, and specific methylation profiling. The device performance was evaluated with a target DNA that harbors a sequence of the methylguanine-DNA methyltransferase, a promoter of glioblastoma multiforme, a lethal brain tumor. The results show that we successfully profiled the methylated and nonmethylated forms at picomolar concentrations. Further, fluorescence kinetics and molecular dynamics simulations revealed that the position of the methylation site(s), their proximity, and accessibility to the toe-hold region of the tweezer probe are the primary determinants of the device performance.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acsnano.9b10085 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A multi-omics Mendelian randomization identifies putatively causal genes and DNA methylation sites for asthma.
World Allergy Organ J
December 2024
Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang, China.
Background: Asthma is a global chronic respiratory disease with complex pathogenesis. While current therapies offer some relief, they often fall short in effectively managing symptoms and preventing exacerbations for numerous patients. Thus, understanding its mechanisms and discovering new drug targets remains a pressing need for better treatment.
View Article and Find Full Text PDFEditorial: Epigenetic regulation of genome integrity and its implications in human diseases.
Front Cell Dev Biol
December 2024
Institut Curie, PSL Research University, CNRS UMR3244, Dynamics of Genetic Information, Sorbonne Université, Paris, France.
BnaHSFA2, a heat shock transcription factor interacting with HSP70 and MPK11, enhances freezing tolerance in transgenic rapeseed.
Plant Physiol Biochem
December 2024
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China. Electronic address:
Heat shock transcription factors (Hsfs) play important roles in plant developmental regulations and various abiotic stress responses. However, their evolutionary mechanism of freezing tolerance remains poorly understood. In our previous transcriptomics study based on DNA methylation sequencing, the BnaHsfA2 was found to be significantly accumulated in winter rapeseed (Brassica rapa L.
View Article and Find Full Text PDFAtlas-scale single-cell DNA methylation profiling with sciMETv3.
Cell Genom
December 2024
Department of Molecular & Medical Genetics, Oregon Health & Science University, Portland, OR, USA; Cancer Early Detection Advanced Research Institute, Oregon Health & Science University, Portland, OR, USA; Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR, USA; Knight Cancer Institute, Oregon Health & Science University, Portland, OR, USA. Electronic address:
Single-cell methods to assess DNA methylation have not achieved the same level of cell throughput per experiment compared to other modalities, with large-scale datasets requiring extensive automation, time, and other resources. Here, we describe sciMETv3, a combinatorial indexing-based technique that enables atlas-scale libraries to be produced in a single experiment. To reduce the sequencing burden, we demonstrate the compatibility of sciMETv3 with capture techniques to enrich regulatory regions, as well as the ability to leverage enzymatic conversion, which can yield higher library diversity.
View Article and Find Full Text PDFThe methylome of clonal seagrass shoots shows age-associated variation and differentiation of roots from other tissues.
Biochim Biophys Acta Gen Subj
December 2024
Algal and Microbial Biotechnology Division, Faculty of Biosciences and Aquaculture, Nord University, Universitetsalléen 11, 8026 Bodø, Norway.
Factors influencing variance of DNA methylation in vegetatively reproducing plants, both terrestrial plants and aquatic seagrasses, is just beginning to be understood. Improving our knowledge of these mechanisms will increase understanding of transgenerational epigenetics in plant clones, of the relationship between DNA methylation and seagrass development, and of the drivers of epigenetic variation, which may underly acclimation in clonally reproducing plants. Here, we sampled leaves, rhizomes and roots of three physically and spatially separated ramet sections from a clonally propagated field of the seagrass Zostera marina.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!