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Integrating cellular electron microscopy with multimodal data to explore biology across space and time.
Cell
February 2024
Biozentrum, University of Basel, Spitalstrasse 41, 4056 Basel, Switzerland. Electronic address:
Biology spans a continuum of length and time scales. Individual experimental methods only glimpse discrete pieces of this spectrum but can be combined to construct a more holistic view. In this Review, we detail the latest advancements in volume electron microscopy (vEM) and cryo-electron tomography (cryo-ET), which together can visualize biological complexity across scales from the organization of cells in large tissues to the molecular details inside native cellular environments.
View Article and Find Full Text PDFPupillometry as a new window to player fatigue? A glimpse inside the eyes of a Euro Cup Women's Basketball team.
Biol Sport
January 2024
Research Center for High Performance Sport, Universidad Católica de Murcia, Murcia, Spain.
A rapidly emerging area of interest in high-pressure environments is that of pupillometry, where handheld quantitative infrared pupillometers (HQIPs) are able to track psycho-physiological fatigue in a fast, objective, valid, reliable, and non-invasive manner. However, the application of HQIPs in the context of athlete monitoring is yet to be determined. Therefore, the main aim of this pilot study was to examine the potential usefulness of a HQIP to monitor game-induced fatigue inside a professional female basketball setting by determining its (1) test-retest repeatability, (2) relationship with other biomarkers of game-induced fatigue, and (3) time-course from rested to fatigued states.
View Article and Find Full Text PDFFungal community inside lichen: a curious case of sparse diversity and high modularity.
Environ Microbiome
October 2023
Korean Lichen Research Institute, Sunchon National University, 255 Jungang-ro, Suncheon, 57922, South Korea.
Article Synopsis
- Lichens are made up of fungi and tiny plants that work together, and they also have other fungi living inside them called endolichenic fungi, which help the lichen survive in different ways.
- Researchers studied two types of lichens and found out that the endolichenic fungi are less diverse and different from the fungi that live on the bark of trees nearby.
- The endolichenic fungi have a simpler structure compared to the surrounding fungi, which helps scientists understand how these tiny ecosystems work.
Candidatus Nemesobacterales is a sponge-specific clade of the candidate phylum Desulfobacterota adapted to a symbiotic lifestyle.
ISME J
November 2023
Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708WE, Wageningen, The Netherlands.
Members of the candidate phylum Dadabacteria, recently reassigned to the phylum Candidatus Desulfobacterota, are cosmopolitan in the marine environment found both free-living and associated with hosts that are mainly marine sponges. Yet, these microorganisms are poorly characterized, with no cultured representatives and an ambiguous phylogenetic position in the tree of life. Here, we performed genome-centric metagenomics to elucidate their phylogenomic placement and predict the metabolism of the sponge-associated members of this lineage.
View Article and Find Full Text PDFAn updated literature on BRAF inhibitors (2018-2023).
Mol Divers
August 2024
Department of Pharmaceutical Chemistry, Integrated Drug Discovery Centre, Acharya & BM Reddy College of Pharmacy, Bengaluru, Karnataka, India.
BRAF is the most common serine-threonine protein kinase and regulates signal transduction from RAS to MEK inside the cell. The BRAF is a highly active isoform of RAF kinase. BRAF has two domains such as regulatory and kinase domains.
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