The effect of solvent species and quantity of solvent used for spreading on the morphology of Langmuir monolayer composed of palmitic and lignoceric acids was investigated based on atomic force microscopy observations. The variation in domain size depending on the evaporation time of the spreading solution indicated that the mixed monolayer was in a non-equilibrium phase-separated state.
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Diffusiophoresis promotes phase separation and transport of biomolecular condensates.
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A molecular segregation inside a nanoparticle was crucial for its properties but usually hard to be determined, especially for organic particles. Herein, non-equilibrium polymeric nanoparticles loading a luminogen via an aggregation-induced emission (AIE) were prepared via an instant formation process, flash nanoprecipitation (FNP). Small organic molecules, polymeric excipients, and oily compounds were used as coprecipitants to reveal effects of conjugate moiety, glass transition temperature (T), and a condensed state of a coprecipitant on the fluorescence (FL) intensity of the suspension.
View Article and Find Full Text PDFDiffusiophoresis promotes phase separation and transport of biomolecular condensates.
Res Sq
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Department of Mechanical and Aerospace Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260.
The internal microenvironment of a living cell is heterogeneous and comprises a multitude of organelles with distinct biochemistry. Amongst them are biomolecular condensates, which are membrane-less, phase-separated compartments enriched in system-specific proteins and nucleic acids. The heterogeneity of the cell engenders the presence of multiple spatiotemporal gradients in chemistry, charge, concentration, temperature, and pressure.
View Article and Find Full Text PDFDiffusiophoresis promotes phase separation and transport of biomolecular condensates.
bioRxiv
February 2024
Department of Mechanical and Aerospace Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260.
The internal microenvironment of a living cell is heterogeneous and comprises a multitude of organelles with distinct biochemistry. Amongst them are biomolecular condensates, which are membrane-less, phase-separated compartments enriched in system-specific proteins and nucleic acids. The heterogeneity of the cell engenders the presence of multiple spatiotemporal gradients in chemistry, charge, concentration, temperature, and pressure.
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