AI Article Synopsis
- * This study focused on the role of deregulated microRNAs (miRNAs) in LCa tissues from patients with and without lymph node metastases, identifying a significant reduction in miR-449a in those with metastases.
- * The research suggests that miR-449a could be a potential therapeutic target, as its overexpression in LCa cells inhibited their invasive behavior and slowed cell growth, indicating its promise as a biomarker for diagnosis and treatment of LCa.
Article Abstract
Laryngeal cancer (LCa), a neoplasm of the head and neck region, is a leading cause of death worldwide. Surgical intervention remains the mainstay of LCa treatment, but a crucial point is represented by the possible nodal involvement. Therefore, it is urgently needed to develop biomarkers and therapeutic tools able to drive treatment approaches for LCa. In this study, we investigated deregulated microRNAs (miRNAs) in tissues from LCa patients with either lymph node metastases (N+) or not (N-). miRNA expression profiling was performed by a comprehensive PCR array and subsequent validation by RT-qPCR. Results showed a significant decrease of miR-449a expression in N+ compared to N- patients, and miR-133b down-modulation in LCa tissues compared to paired normal ones. Receiver operating characteristic (ROC) curve analysis revealed the potential diagnostic power of miR-133b for LCa detection. According to the validation results, we selected miR-449a for further in vitro studies. Ectopic miR-449a expression in the LCa cell line Hep-2 inhibited invasion and motility in vitro, slowed cell proliferation, and induced the downregulation of Notch1 and Notch2 as direct targets of miR-449a. Collectively, this study provides new promising biomarkers for LCa diagnosis and a new opportunity to use miR-449a for the treatment of nodal metastases in LCa patients.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218231 | PMC |
| http://dx.doi.org/10.1016/j.omtn.2020.04.006 | DOI Listing |
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