Retinal photoreceptors targeting SA--AA coated multilamellar liposomes carrier system for cytotoxicity and cellular uptake evaluation.

Here, the retinal targeting SA-g-AA coated multilamellar liposomes carrier synthesized to deliver the bioactive agents into the retinal region of the eye. The multilayered targeting macromolecules of liposomes prepared using a layer-by-layer assembly. The curcumin (CUR) and Rhodamine B (RhB) dyes loaded in a multilamellar vesicle (MLV) were synthesised by the lipid film hydration method. The sodium alginate grafted acrylic acid (SA--AA) conjugated with riboflavin (RB) was coated over MLV by O/W emulsion method followed by ionotropic gelation. FT-IR and H NMR spectroscopy techniques used to analyse the structural features of the MLV-SA--AA-RB. The results of DLS and TEM revealed that the carrier could be of uniform spheres, with a low polydispersity index, and outstanding performance in phrases of dye encapsulation and extended-release ability. An MTT assay investigated cell viability against Fibroblast WS1, and human embryonic stem cells-derived retinal pigment epithelial cells (hESC-RPE) implied that the carrier is of excellent biocompatibility. Retina targeting nature of the system confirmed via cellular uptake results revealed that the increases the dye concentration in the cells. Overall, the outcomes suggested that carriers could lead to the improvement of a feasible two photoreceptors targeting drug carriers, and it has the potential to deliver the multidrug in the retinal region of the eye.