DNA double-strand breaks (DSBs) could be deleterious and lead to age-related diseases, such as cancer. Recent evidence, however, associates DSBs with vital cellular processes. As discussed here, genome-wide mapping of DSBs revealed an unforeseen coupling mechanism between transcription and DNA repair at super-enhancers, as means of hypertranscription of oncogenic drivers.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7199745 | PMC |
| http://dx.doi.org/10.1080/23723556.2019.1698933 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Common occurrence of hotspots of single strand DNA breaks at transcriptional start sites.
BMC Genomics
April 2024
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, 361102, Xiamen, China.
Background: We recently developed two high-resolution methods for genome-wide mapping of two prominent types of DNA damage, single-strand DNA breaks (SSBs) and abasic (AP) sites and found highly complex and non-random patterns of these lesions in mammalian genomes. One salient feature of SSB and AP sites was the existence of single-nucleotide hotspots for both lesions.
Results: In this work, we show that SSB hotspots are enriched in the immediate vicinity of transcriptional start sites (TSSs) in multiple normal mammalian tissues, however the magnitude of enrichment varies significantly with tissue type and appears to be limited to a subset of genes.
Genome-wide profiles of DNA damage represent highly accurate predictors of mammalian age.
Aging Cell
May 2024
State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, China.
Article Synopsis
- Researchers are focusing on finding new age-related biomarkers, especially through DNA damage, but existing methods haven't been precise enough for genome-wide studies.
- Two new techniques for mapping DNA damage at a detailed level were developed, allowing for a better understanding of single-strand breaks and abasic sites.
- Using a mouse model, the study found that genomic patterns of DNA damage can predict age more accurately than traditional transcriptome analysis, suggesting these patterns could be valuable for both practical applications and scientific research.
Mapping the breakome reveals tight regulation on oncogenic super-enhancers.
Mol Cell Oncol
February 2020
The Lautenberg Center for General and Tumor Immunology; Department of Immunology and Cancer Research-IMRIC, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
DNA double-strand breaks (DSBs) could be deleterious and lead to age-related diseases, such as cancer. Recent evidence, however, associates DSBs with vital cellular processes. As discussed here, genome-wide mapping of DSBs revealed an unforeseen coupling mechanism between transcription and DNA repair at super-enhancers, as means of hypertranscription of oncogenic drivers.
View Article and Find Full Text PDFThe DNA double-strand "breakome" of mouse spermatids.
Cell Mol Life Sci
August 2018
Department of Biochemistry, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada.
De novo germline mutations arise preferentially in male owing to fundamental differences between spermatogenesis and oogenesis. Post-meiotic chromatin remodeling in spermatids results in the elimination of most of the nucleosomal supercoiling and is characterized by transient DNA fragmentation. Using three alternative methods, DNA from sorted populations of mouse spermatids was used to confirm that double-strand breaks (DSB) are created in elongating spermatids and repaired at later steps.
View Article and Find Full Text PDFMapping DNA Breaks by Next-Generation Sequencing.
Methods Mol Biol
June 2018
Laboratory of Pathology, NCI/NIH, Bethesda, MD, 20892, USA.
Here, we present two approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) in the genome of human cells. We named these methods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!