27 ejaculates were incubated together with polyvidone iodine diluted with physiological saline solution in various proportions. 10 minutes, 1 hour, 4 hours, and 20 hours later, we determined the percentages of mobile and viable spermatozoa in both the native and the incubated seminal fluid. 1% polyvidone iodine solution was totally spermicidal already after 10 minutes of incubation. 0.1% solution only slightly reduced the sperm motility and viability. 0.01% polyvidone iodine solution even resulted in a temporary increase of motility.
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