Magnetic dual-template molecularly imprinted polymer based on syringe-to-syringe magnetic solid-phase microextraction for selective enrichment of p-Coumaric acid and ferulic acid from pomegranate, grape, and orange samples.

Magnetic dual-template molecularly imprinted polymer (FeO@SiO-MDMIP) was prepared to enrich and determine both p-Coumaric acid (p-CA) and ferulic acid (FA) based on syringe-to-syringe magnetic solid-phase microextraction (SS-MSPME). The obtained MDMIP was characterized and recognized, and then its adsorbing performance was studied. Based on the results, the FeO@SiO-MDMIP indicated selective recognition towards p-CA and FA with large adsorption capacity. The optimization of MDMIP-SS-MSPME conditions (pH, FeO@SiO-MDMIP mass, NaCl concentration, number of cycle, and elution volume) were conducted using the central composite design (CCD). Under the optimum conditions, an effectual and a convenient method was established to determine p-CA and FA in pomegranate, grapes, and orange samples based on SS-MSPME coupling with high-performance liquid chromatography-ultraviolet (HPLC-UV). Our developed method showed the limit of detection (LOD) of 0.08 ng mL for p-CA and 0.07 ng mL for FA. The method also indicated good linearity with R > 0.99 and good recoveries of 85.12-94.96% with RSDs ≤ 5.58% spiked at three various concentration levels in pomegranate, grapes, and orange samples.