Simultaneous eco-toxicity assessment technique using an online monitoring system: effects of different environmental factors on swimming behavior of zebrafish (Danio rerio).

Environmental factors, such as photoperiod and temperature were the main limiting factors for the survival of organisms in the nature environment. Changes in environmental factors are well predicted but determining their effects on organisms are challenging hot topic in the field of eco-toxicology. Thus, technology based eco-toxicity assessment was focused worldwide. In this research, the effects of different temperatures (15 °C, 22 °C, 30 °C, 32 °C, and 35 °C) and photoperiods (dark and light periods) on the continuous behavior responses of Zebrafish (Danio rerio) were investigated using an online monitoring system (OMS). We designed a new fish chamber with sensors to measure the behavior responses of zebrafish under different conditions. Data obtained from the OMS could be assessed for factors such as difference in swimming behavior, circadian rhythm, and avoidance behavior using latest software (MATLAB). The observed behavior anomalies on zebrafish under different temperatures and continuous photoperiods were statically significant (p < 0.05). We conclude that the new designed fish chamber (behavior sensors) is good in sensing behavioral responses of zebrafish under different conditions. The fish behavior strength could be a potential biomarker to assess the effects of environmental factors. The present study would be a basic platform for assessing the effects of different stressors simultaneously on swimming behavior of zebrafish.