Accelerated biomethane production from lignocellulosic biomass: Pretreated by mixed enzymes secreted by Trichoderma viride and Aspergillus sp.

Biological pretreatment is a promising technology to increase biogas yield. The methane yield and microbial community resulting from anaerobic digestion of maize straw after pretreatment of enzymes [extracted from Trichoderma viride (ETv) and Aspergillus sp. (EAs)] at different mixing ratios [5/0, 4/1, 3/2, 2/3, 1/4, 0/5] were evaluated. The methane yields from mixed enzymes pretreatment were higher than single enzymes pretreatments of ETv and EAs. The optimal enzymes mixing ratio of ETv and EAs was found to be 2/3, with the cumulative methane yield 512.64 mL/g TS, which was 31.74% higher than the control. Enzymatic pretreatment promoted an increase in the abundance of bacteria and archaea associated with cellulose decomposition. The majority of bacteria and archaea were assigned to Bacteroidetes, Firmicutes and Methanosaeta.