sp. nov., an endophytic actinomycete isolated from the root of in Thailand.

An endophytic actinomycete, strain 3MP-10, isolated from the root of was taxonomically studied based upon polyphasic approaches. This strain formed spiral spore chains on aerial mycelia. ll-Diaminopimelic acid, glucose and ribose were found in the whole-cell hydrolysates. It belonged to the genus and was closely related to DSM 42101 (98.9 %) and JCM 16909 (98.6 %) based on 16S rRNA gene sequence analysis results. The major menaquinones were MK-10(H), MK-10(H) and MK-9(H). The predominant cellular fatty acids were isoC, anteiso-C and anteisoC. The detected phospholipids were diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phosphatidylethanolamine and phosphatidylglycerol. Strain 3MP-10 had a genome size of 7.2 Mb with a genome G+C content of 73.4 mol%. Results of genome-based similarity analysis revealed ANIb values of 84.94 and 84.77 %, ANIm values of 88.01 and 87.92 %, and dDDH values of 29.9 and 29.6 % when compared with DSM 42101 and JCM 16909, respectively. Based on the polyphasic approach, digital DNA-DNA relatedness and average nucleotide identity, we propose that the novel actinomycete represents a novel species, , with type strain 3MP-10 (=JCM 33328=TISTR 2646).