Anti-inflammatory ergosterol derivatives from the endophytic fungus .

Two new ergosterol derivatives namely, chlamydosterols A [(22,24)-ergosta-7,22-diene-3β,5α,6β-triol 6-decanoate] () and B [5α,6β,25-trihydroxy-(22,24)-ergosta-7,22-dien-3-one] ( and three known ergosterols: ergosta-7,22-dien-3β-ol (), ergosta-5,7,22-triene-3β-ol (), and ergosta-7,22-diene-3,5α,6β-triol ( were separated from the EtOAc extract of the endophytic fungus isolated from (Asteraceae) leaves growing in Saudi Arabia. Their structural assignment was accomplished by various spectroscopic analyses, as well as comparing with the published data. The 5-lipoxygenase (5-LOX) inhibitory potential of the isolated metabolites was assessed. Compounds and displayed moderate 5-LOX inhibitory potential with ICs 3.06 and 3.57 µM, respectively compared to indomethacin (IC 1.13 µM).