Study on species in the Bulgarian flora.

Heliyon

Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97331, USA.

Published: December 2019

Genus (Amaryllidaceae) includes 19 species in Europe and the Middle East. The Flora of Bulgaria recognizes two species: L. and Hook. is characterized by relatively high morphological variability, leading some authors to identify some populations as Celak. However, the occurrence of in the Bulgarian flora is disputed. The hypothesis was that populations previously identified as belong indeed to a separate species. Therefore, the objective of this study was to compare and with plants from populations previously identified as . Morphological, DNA, embryological and anatomical analyzes were conducted to meet the objective. The morphological characteristics and DNA dendrogram revealed that and were situated in the same cluster and had significant morphological similarity, whereas plants from populations identified as were dissimilar in morphology and situated in a separate cluster. The revealed features of the generative sphere showed similarities across the species. Scanning electron microscopy (SEM) analyses of the surface revealed that the anticillinal walls of and were straight, while those of were wavy. This research demonstrated that the plants of and those from populations identified as are morphologically, embryologically and genetically similar, thus refuting the hypothesis. This study did not provide sufficient evidence to support the claim of the existence of in the Bulgarian flora; the populations identified as in Bulgaria may be forms of .

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191607PMC
http://dx.doi.org/10.1016/j.heliyon.2019.e03021DOI Listing

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