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High-throughput specificity profiling of antibody libraries using ribosome display and microfluidics.
Cell Rep Methods
December 2024
GigaGen, Inc. (a Grifols company), San Carlos, CA, USA. Electronic address:
In this work, we developed PolyMap (polyclonal mapping), a high-throughput method for mapping protein-protein interactions. We demonstrated the mapping of thousands of antigen-antibody interactions between diverse antibody libraries isolated from convalescent and vaccinated COVID-19 donors and a set of clinically relevant SARS-CoV-2 spike variants. We identified over 150 antibodies with a variety of distinctive binding patterns toward the antigen variants and found a broader binding profile, including targeting of the Omicron variant, in the antibody repertoires of more recent donors.
View Article and Find Full Text PDFRetrospective Analysis of Coronavirus SARS-CoV-2 Antibody Levels in COVID-19 Convalescent Plasma From Blood Donors.
Can J Infect Dis Med Microbiol
December 2024
Institute of Transfusion Medicine, Blood Center of Zhejiang Province, Jianye Road 789, Hangzhou, Zhejiang 310052, China.
To detect and analyze coronavirus SARS-CoV-2 antibody levels in convalescent plasma from donors who have recovered from COVID-19. Plasma samples from 88 donors aged 20-54 years who were diagnosed with COVID-19 and who were eligible to donate from Zhejiang Province, China, were collected as the experimental group, and 56 samples from healthy blood donors were used as controls. Anti-SARS-CoV-2 antibodies, including Ab and IgM, were detected via chemiluminescent immunoassay, and neutralizing antibodies were measured via the microneutralization method.
View Article and Find Full Text PDFAnalysis of antigen specificity of Treg cell clonotypes expanded upon SARS-CoV-2 infection.
Int Immunol
December 2024
Laboratory of Molecular Immunology, Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan.
The pandemic outbreak of SARS-CoV-2 has threatened human health worldwide. Among protective immune reactions, T cell responses are diverse among individuals, which is related to the differences in severity. A T cell subset, regulatory T (Treg) cells, is crucial for limiting excessive immune responses.
View Article and Find Full Text PDFDevelopment of a nomogram for high antibody titre of COVID-19 convalescent plasma.
Epidemiol Infect
December 2024
Department of Blood Transfusion, First Affiliated Hospital, Third Military Medical University (Army Medical University), Chongqing, P.R. China.
This study aimed to develop a predictive tool for identifying individuals with high antibody titers crucial for recruiting COVID-19 convalescent plasma (CCP) donors and to assess the quality and storage changes of CCP. A convenience sample of 110 plasma donors was recruited, of which 75 met the study criteria. Using univariate logistic regression and random forest, 6 significant factors were identified, leading to the development of a nomogram.
View Article and Find Full Text PDFCreating a plasma coordination center to support COVID-19 outpatient trials across a national network of hospital blood banks.
J Clin Transl Sci
November 2024
Department of Neurology, BIOS Clinical Trial Coordinating Center, Trial Innovation Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Introduction: In response to the COVID-19 pandemic, we rapidly implemented a plasma coordination center, within two months, to support transfusion for two outpatient randomized controlled trials. The center design was based on an investigational drug services model and a Food and Drug Administration-compliant database to manage blood product inventory and trial safety.
Methods: A core investigational team adapted a cloud-based platform to randomize patient assignments and track inventory distribution of control plasma and high-titer COVID-19 convalescent plasma of different blood groups from 29 donor collection centers directly to blood banks serving 26 transfusion sites.
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