Objective: To investigate the molecular characteristics of carbapenem-resistant (CRE) from county hospitals in China.
Materials And Methods: Forty-three sequential non-duplicate CRE strains (including 33 isolates, 4 isolates, 3 isolates, 1 , 1 and 1 ) were collected from 4 county hospitals and 2 municipal hospitals. Antimicrobial susceptibility testing was conducted by broth microdilution method, using 3-aminophenylboronic acid and EDTA and the modified carbapenem inactivation method (mCIM) to screen phenotype of carbapenemase. β-Lactamases were characterized by polymerase chain reaction (PCR) and DNA sequencing. The transferability of was investigated by transformation experiment. Clonal relatedness was evaluated by pulsed-field gel electrophoresis and multilocus sequence typing .
Results: The results of antimicrobial susceptibility testing indicated that 43 CRE strains were resistant to most of the antimicrobial agents, except tigecycline and colistin. Overall, 93%, 93%, and 97.7% of these strains were resistant to imipenem, meropenem, and ertapenem, respectively. PCR and DNA sequencing indicated that 67.4% (29/43) were positive isolates, in which 3.4% (1/29) was coproduced with . In addition, 7.0% (3/43), 4.7% (2/43), 4.7% (2/43), 2.3% (1/43), 2.3% (1/43) were , , , , positive isolates, respectively. The 29 -positive isolates belonged to 12 different PFGE type and designated as ST11 (n=20) and ST15, ST39, ST116, ST667, ST2245, ST2338. The plasmid bearing could be transferred into recipient J53 through transformation.
Conclusion: Our study indicated the dissemination of CRE between the tertiary hospitals and secondary hospitals.
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| http://dx.doi.org/10.2147/IDR.S248147 | DOI Listing |
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