AI Article Synopsis

  • The study aims to understand the role of IL-31 and its receptor IL-31RA in promoting fibrosis in scleroderma, a condition affecting the skin and lungs.
  • Researchers measured IL-31 levels in plasma and tissues from scleroderma patients and conducted experiments on mice and fibroblast cells to analyze the effects of IL-31.
  • Results indicated high levels of IL-31 in some patients, linked to changes in fibroblast behavior that promote fibrosis, suggesting that the IL-31/IL-31RA pathway could be a potential target for treatment.

Article Abstract

Objectives: Cytokines released by infiltrating T cells may promote mechanisms leading to fibrosis in scleroderma. The aim of this study was to investigate the role of the Th2 cytokine IL-31, and its receptor IL-31RA, in scleroderma skin and lung fibrosis.

Methods: IL-31 was measured by ELISA of plasma, and by immunochemistry of fibrotic skin and lung tissue of scleroderma patients. The receptor, IL-31RA, was assayed by qPCR of tissue resident cells. Next-generation sequencing was used to profile the responses of normal skin fibroblasts to IL-31. In wild-type Balb/c mice, IL-31 was administered by subcutaneous mini pump, with or without additional TGFβ, and the fibrotic reaction measured by histology and ELISA of plasma.

Results: IL-31 was present at high levels in plasma and fibrotic skin and lung lesions in a subset of scleroderma patients, and the receptor overexpressed by downstream cells relevant to the disease process, including skin and lung fibroblasts, through loss of epigenetic regulation by miR326. In skin fibroblasts, IL-31 induced next generation sequencing profiles associated with cellular growth and proliferation, anaerobic metabolism and mineralization, and negatively associated with angiogenesis and vascular repair, as well as promoting phenotype changes including migration and collagen protein release via pSTAT3, resembling the activation state in the disease. In mice, IL-31 induced skin and lung fibrosis. No synergy was seen with TGFβ, which supressed IL-31RA.

Conclusion: IL-31/IL-31RA is confirmed as a candidate pro-fibrotic pathway, which may contribute to skin and lung fibrosis in a subset of scleroderma patients.

Download full-text PDF

Source
http://dx.doi.org/10.1093/rheumatology/keaa195DOI Listing

Publication Analysis

Top Keywords

skin lung
28
lung fibrosis
12
scleroderma patients
12
skin
9
fibrosis scleroderma
8
receptor il-31ra
8
fibrotic skin
8
patients receptor
8
skin fibroblasts
8
fibroblasts il-31
8

Similar Publications

is a human pathogen responsible for a variety of diseases, from skin, soft tissue, and lung infections to severe cases such as meningitis, infective endocarditis, and bacteremia. The high level of antibiotic resistance in these pathogens, exemplified by methicillin-resistant (MRSA), necessitates the development of effective antibiotics. Thus, this work introduced the chemical synthesis of ethyl 3,5-dibromoorsellinate, a derivative of ethyl orsellinate from the lichen mycobiont of , and its effectiveness against MRSA was assessed.

View Article and Find Full Text PDF

Objective: To characterize the epidemiological characteristics of malignancy in Chinese patients with rheumatoid arthritis (RA) American patients and investigate their associated factors.

Methods: Data were collected from a real-world Chinese RA population and American patients with RA from the National Health and Nutritional Examination Survey. The prevalence and subtypes of malignancy and their potential associated factors were investigated in both populations.

View Article and Find Full Text PDF

Background: Serpentine supravenous hyperpigmentation (SSH) is known as a phenomenon occurring during the infusion of chemotherapy agents in the underlying veins. Chemotherapy agents have potential to cause infusion reactions when used systematically. Linear hyperpigmentation and reticular hyperpigmentation are the differential diagnosis for this phenomenon.

View Article and Find Full Text PDF

Nailfold videocapillaroscopy findings and associations with organ involvement in mixed connective tissue disease.

Clin Exp Rheumatol

December 2024

Service de Rhumatologie, Hôpital Cochin, AP-HP Centre Université Paris Cité, Paris, and INSERM U1016, Institut Cochin, CNRS UMR8104, Paris, France.

Objectives: To investigate nailfold videocapillaroscopy (NVC) abnormalities in mixed connective tissue disease (MCTD).

Methods: Patients with MCTD followed at the Rheumatology Department in Cochin Hospital (Paris, France) were identified based on individual record review. Diagnosis of MCTD required fulfillment of one of the three sets of classification criteria.

View Article and Find Full Text PDF

A novel clinical brain prognostic index for KRAS-mutated lung cancer and brain metastases (KRAS-BPI): Real-world evidence from two large European centers.

Lung Cancer

December 2024

Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden; Medical Unit Head & Neck, Lung and Skin Cancer, Theme Cancer, Karolinska University Hospital and Comprehensive Cancer Center, Stockholm, Sweden; Thoracic Oncology Center, Theme Cancer, Karolinska University Hospital and Comprehensive Cancer Center, Stockholm, Sweden.

Introduction: Several prognostic scores were developed for non-small-cell lung cancer (NSCLC) patients with brain metastases (BM), though limited data reported for the KRAS-mutated subgroup. KRAS-targeted therapies have improved extracranial and intracranial response, highlighting the need for reliable prognostic biomarkers.

Methods: A retrospective cohort (2010-2020) comprising 220 patients with BM KRAS-mutated NSCLC from two large academic Thoracic Oncology centers (Karolinska and Heidelberg) was analyzed.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!