Neural crest cells possess characteristics of stem cells including plasticity and ability to differentiate into various cell types. HNK1 and Sox10 are markers of neural crest cell progenitors that have been demonstrated in osteoblasts during osteogenesis of the maxilla and mandible. We investigated the presence of Sox10 and HNK1 during regeneration of mandibular bone defects. Defects were created in mandibles of rats. Samples of these defects were collected at 7, 14 and 28 days post-surgery; bone regeneration was observed during this period. Immunohistochemical analysis revealed expression of HNK1 and Sox10 in osteoblasts, osteocytes and osteogenic cells, whereas osteoclasts were unstained. HNK1 expression was increased in osteoblasts and osteocytes over time and SOX10 expression was found in osteoblasts and osteogenic cells at 7, 14 and 28 days post-surgery. HNK1 and Sox10 are present in osteoblasts, osteocytes and osteogenic cells during mandible bone regeneration.
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