species are the leading cause of gastroenteritis worldwide and an emerging threat in developing countries. Here, we report the draft whole-genome sequences of 51 and 12 strains isolated from patients with gastroenteritis in Santiago, Chile.
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http://dx.doi.org/10.1128/MRA.00072-20 | DOI Listing |
Pathogens
January 2025
Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan.
While studying the prevalence and profile of antibiotic resistance among isolated from the feces of calves with signs of colibacillosis, a strain with a wide spectrum of drug resistance was isolated. Whole-genome sequencing, followed by bioinformatic processing and the annotation of genes of this strain, showed that the genome has a total length of 4,803,482 bp and contains 4986 genes, including 122 RNA genes. A total of 31% of the genes are functionally significant and represent 26 functional groups.
View Article and Find Full Text PDFAntibiotics (Basel)
January 2025
Clinical Microbiology Laboratory, Miguel Servet University Hospital, 50009 Zaragoza, Spain.
: Infections caused by multidrug-resistant (MDR)bacteria pose a significant public health threat by worsening patient outcomes, contributing to hospital outbreaks, and increasing health and economic burdens. Advanced genomic tools enhance the detection of resistance genes, virulence factors, and high-risk clones, thus improving the management of MDR infections. In the Autonomous Community of Aragon, the diversity and incidence of carbapenemase-producing Enterobacteriaceae (CPE) have increased during the last years.
View Article and Find Full Text PDFMicrobiol Resour Announc
January 2025
Industrial Genomics Laboratory, FEMSA Biotechnolgy Center, School of Engineering and Sciences, Tecnológico de Monterrey, Monterrey, México.
sp. CC302I was isolated from a highly oligothrophic environment. High-throughput screening shows high codon-readthrough activity for the isolate with no canonical biosynthetic gene cluster responsible.
View Article and Find Full Text PDFMicrobiol Resour Announc
January 2025
Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, India.
The draft genome sequence of DS1 was isolated for high-acidity vinegar fermentation. The DS1 draft genome has a length of 8,793,442 bp, with 44.27% GC content, 3,053 proteins, 3,169 genes, and 15 genes that play an important role in acetic acid production.
View Article and Find Full Text PDFCell Rep Med
January 2025
Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku 160-8582, Tokyo, Japan; Department of Clinical Regenerative Medicine, Fujita Medical Innovation Center, Fujita Health University, Ota-ku, Tokyo 144-0041, Japan. Electronic address:
A first-in-human investigator-initiated clinical study of a corneal endothelial cell substitute (CLS001) derived from a clinical-grade induced pluripotent stem cell (iPSC) line shows improvement of visual acuity and corneal stromal edema, with no adverse events for up to 1 year after surgery for the treatment of bullous keratopathy. While preclinical tests, including multiple whole-genome analysis and tumorigenicity tests adhering to the Food and Drug Administration (FDA) draft guidelines, are negative, an additional whole-genome analysis conducted on transplanted CLS001 cells reveals a de novo in-frame deletion of exon22 in the EP300 gene. No adverse events related to the mutation are observed.
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