To investigate plasmid-borne colistin resistance mechanism (plasmid-mediated colistin resistance []) in of human, veterinary, and environmental origin in Bangladesh. A total of 810 samples were collected from different sources. Isolation and identification of was performed using classical bacteriology and then tested for antimicrobial susceptibility. Colistin-resistant isolates were further analyzed for gene using PCR. Minimum inhibitory concentration (MIC) was determined using microbroth dilution technique. After sequencing of gene, phylogenetics was conducted to see the relationship with other gene sequences. A total of 358 were isolated from 810 samples of humans, animals, environment, and food in Bangladesh. Of them 49 (15.9%) isolates were phenotypically resistant to colistin and 254 (70.9%) were resistant to multiple antimicrobials. gene was detected in three isolates of poultry source. For the three positive isolates the MIC of colistin sulfate was 4, 8, and 128 μg/mL. Gene sequencing of two of the three positive isolates and phylogenetic analysis showed close similarities of one isolate to other sequences available at GenBank while the other appeared to have evolved locally. First-ever report on circulation of of livestock origin in Bangladesh.
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