The remediation of metal-polluted water using bacterial biofilms is a promising technology. In order to help its development, the present study aims to evaluate the feasibility to utilize XRF spectrometry for accurate and rapid measurement of metal concentrations in bacterial biofilms used in treatment plants. For that purpose, an ED-XRF spectrometer was used to measure Cd, Cu, Fe, Mn, Ni and Zn concentrations within a matrix of marine bacteria BA3SM1 and its metabolites. Contaminated and control cultures of the strain BA3SM1 were dried and crushed, then analysed by ED-XRF. The LOD value of the analysed metals was between 2.08 and 10.5 µg g. Metal concentrations were also measured by ICP-AES or ICP-MS to support ED-XRF results. The two techniques showed a good linear correlation with a slope of at least 0.949 and of at least 0.985. These results confirm the possibility to measure metal contents by ED-XRF in bacterial matrices.
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