AI Article Synopsis
- Human mesenchymal stem cells (hMSCs) show potential for treating pulmonary fibrosis, but their behavior post-transplantation remains unclear.
- A dual-labeling strategy using bioluminescence imaging (BLI) and near-infrared-persistent luminescence (NIR-PL) allows researchers to visualize transplanted hMSCs effectively.
- This technique enables tracking the movement and survival of these stem cells in real-time, enhancing our understanding of their role in treating pulmonary fibrosis.
Article Abstract
Human mesenchymal stem cells (hMSCs) are promising in the treatment of pulmonary fibrosis (PF). However, the behavior of hMSCs after transplantation, including dynamic translocation, location and survival, impeding the clinical application of hMSCs in PF is still ambiguous. Herein, we report an effective dual-labeling strategy combining endogenous bioluminescence imaging (BLI) and exogenous near-infrared-persistent luminescence (NIR-PL) imaging for in situ visualization of the transplanted stem cells. The long persistent luminescence nanoparticles (LPLNPs), ZnGaGeO:Cr,Eu, were developed to track the dynamic translocation, position and distribution of the transplanted hMSCs, taking advantage of their long-lasting NIR-PL imaging ability and minimal autofluorescence background interference. Moreover, in virtue of their ability to express red-emitting firefly luciferase (RfLuc), the living stem cells after transplantation could be discriminated from the dead cells by BLI. This facile pattern contributes to the in situ monitoring of stem cells regarding their spontaneous behavior in vivo and therefore deepening our knowledge in the role played by the transplanted hMSCs in PF therapy.
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| http://dx.doi.org/10.1039/d0bm00063a | DOI Listing |
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