AI Article Synopsis
- The study aimed to evaluate the effects of Lactobacillus E6-1 on the growth and apoptosis of the human oral cancer cell line Cal-27.
- The MTT assay indicated that Lactobacillus E6-1 inhibited cell proliferation in a dose-dependent manner, while agarose gel electrophoresis revealed DNA fragmentation indicative of apoptosis.
- Additionally, Western blot analysis showed increased expression levels of cyt-c, caspase-9, and caspase-3, suggesting that Lactobacillus E6-1 effectively induces apoptosis in Cal-27 cells.
Article Abstract
Purpose: To study the effect of Lactobacillus E6-1 on proliferation and apoptosis of human oral cancer cell line Cal-27 in vitro.
Methods: MTT assay was carried out by different concentrations of Lactobacillus E6-1 on Cal-27 to detect the inhibitory effect on cell proliferation, and the effect on DNA was detected by agarose gel electrophoresis. The expression levels of cyt-c, caspase-9 and caspase-3 in each group was detected by Western blot. The data were analyzed with SPSS 21.0 software package.
Results: The results of MTT assay showed that E6-1 had obvious inhibitory effect on Cal-27 in a dose-dependent manner. DNA ladder in Cal-27 cells was induced by different concentrations of E6-1 (10, 20, 40 mg/mL). Compared with blank control group, Western blot results showed that the expression of cyt-c, caspase-9 and caspase-3 increased significantly(P<0.05).
Conclusions: Lactobacillus E6-1 can inhibit the proliferation of Cal-27 and effectively induce apoptosis of tumor cells In vitro.
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