The objective of this study was to evaluate three most common scale-up criteria for Haematococcus pluvialis production from cultivation bottles to 2 and 10 L of stirred tank PBRs. Constant volumetric power input (P/V) was found to be the most suitable criterion for H. pluvialis production. Total carotenoid amount per biomass concentration in 2 L and 10 L stirred tank PBRs were determined to be 4.57 mg/g and 4.77 mg/g, respectively. Antioxidant activity of total carotenoids extracted from H. pluvialis was also higher at constant P/V criterion where 46.91% inhibition rate with a total phenolic content of 11.76 mg gallic acid/L was achieved. Obtained results could be used to expand the bioproduction of H. pluvialis and its extracts in commercial scale.
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http://dx.doi.org/10.1016/j.biortech.2020.123434 | DOI Listing |
Biotechnol Biofuels Bioprod
January 2025
Aquaculture Department, Faculty of Fisheries, Ege University, 35040, Izmir, Türkiye.
For biofuels and nutraceuticals, the green microalga Haematococcus pluvialis (Chlorophyceae) is a prospective source of biomass and lipids. This study examined how biomass production and lipid accumulation were affected by temperature (10 °C, 20 °C, and 30 °C) and potassium nitrate (KNO₃) concentrations (0.41 g/L, 0.
View Article and Find Full Text PDFWorld J Microbiol Biotechnol
January 2025
Key Laboratory of Smart Breeding (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Tianjin Agricultural University, Tianjin, 300392, P.R. China.
Revealing the differences of metabolite profiles of H. pluvialis during hyperaccumulation of astaxanthin under the high salinity and nitrogen deficiency conditions was the key issues of the present study. To investigate the optimum NaCl and NaNO concentration and the corresponding metabolic characteristic related to the astaxanthin accumulation in H.
View Article and Find Full Text PDFBiotechnol Adv
January 2025
Key Laboratory of Coastal Biology and Biological Resource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, Shandong, China. Electronic address:
The global market demand for natural astaxanthin (AXT) is growing rapidly owing to its potential human health benefits and diverse industry applications, driven by its safety, unique structure, and special function. Currently, the alga Haematococcus pluvialis (alternative name H. lacustris) has been considered as one of the best large-scale producers of natural AXT.
View Article and Find Full Text PDFBioengineering (Basel)
November 2024
Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST), Hanoi 10000, Vietnam.
Background: , a green microalga, is a rich source of natural astaxanthin and a potent antioxidant with high commercial value. This study investigates the biological characteristics and potential of HB isolated from Hoa Binh, Vietnam, for growth and astaxanthin accumulation using a two-phase culture method.
Methods: HB was cultured in a C/RM medium at 25 °C, and morphological characteristics were examined.
Methods Mol Biol
December 2024
Bioprotection Aotearoa, School of Food Technology and Natural Sciences, Massey University, Palmerston North, New Zealand.
This chapter describes the protocol for heterologous expression of Phytophthora proteins in the yeast Pichia pastoris. Two methods to prepare the constructs for expression are described, using two different strains of P. pastoris, as well as methods for protein expression and purification by immobilized metal ion affinity (IMAC).
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