AI Article Synopsis
- Lamin C2 is a germ cell-specific protein derived from the lamin A gene, mainly studied in male germ cells but now investigated in female germinal vesicle oocytes.
- The research found LMN C2 localized at the nuclear membrane of oocytes across different mammalian species, confirmed through immunoblotting and GFP tagging.
- Notably, LMN C2 levels dropped significantly in oocytes from older females and vanished during key processes like nuclear envelope breakdown and meiotic maturation, offering fresh insights into its role in oocyte development.
Article Abstract
Lamin C2 (LMN C2) is a short product of the lamin a gene. It is a germ cell-specific lamin and has been extensively studied in male germ cells. In this study, we focussed on the expression and localization of LMN C2 in fully-grown germinal vesicle (GV) oocytes. We detected LMN C2 in the fully-grown germinal vesicle oocytes of various mammalian species with confirmation done by immunoblotting the wild type and Lmnc2 gene deleted testes. Expression of LMN C2 tagged with GFP showed localization of LMN C2 to the nuclear membrane of the oocyte. Moreover, the LMN C2 protein notably disappeared after nuclear envelope breakdown (NEBD) and the expression of LMN C2 was significantly reduced in the oocytes from aged females and ceased altogether during meiotic maturation. These results provide new insights regarding LMN C2 expression in the oocytes of various mammalian species.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7188254 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0229781 | PLOS |
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