AI Article Synopsis
- X-ray phase contrast micro-tomography offers a non-invasive method for 3D imaging of spinal cord morphology, capturing both vascular and neuronal structures simultaneously without contrast agents.
- Most previous studies required removal of the spinal cord from the spinal canal, which limited the resolution of the images.
- This research advances the method by providing high-resolution images of the fixed spinal cord while preserving the surrounding bones, paving the way for potential in-vivo applications.
Article Abstract
A crucial issue in the development of therapies to treat pathologies of the central nervous system is represented by the availability of non-invasive methods to study the three-dimensional morphology of spinal cord, with a resolution able to characterize its complex vascular and neuronal organization. X-ray phase contrast micro-tomography enables a high-quality, 3D visualization of both the vascular and neuronal network simultaneously without the need of contrast agents, destructive sample preparations or sectioning. Until now, high resolution investigations of the post-mortem spinal cord in murine models have mostly been performed in spinal cords removed from the spinal canal. We present here post-mortem phase contrast micro-tomography images reconstructed using advanced computational tools to obtain high-resolution and high-contrast 3D images of the fixed spinal cord without removing the bones and preserving the richness of micro-details available when measuring exposed spinal cords. We believe that it represents a significant step toward the in-vivo application.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7173906 | PMC |
| http://dx.doi.org/10.1364/BOE.386837 | DOI Listing |
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