The Influence of Anionic, Cationic Surfactant and AOT/Water/Heptane Reverse Micelle on Photophysical Properties of Crocin: Compare with RPMI Effect.

Encapsulation of crocin (CN), having large nonlinear optical (NLO) properties, can be utilized in studies of photodynamic therapy (PDT). For this purpose, photo-physical and NLO properties of CN encapsulation with and without cell culture medium (CCM) were investigated. As well, nonlinear absorption (NLA) coefficient and nonlinear refractive (NLR) indices were found to be 10 (cm W) and 10 (cm W); respectively. The results revealed that NLO properties of CN had changed through its dipole moment. Reflecting on the theory of Bilot and Kawski, it was evidenced that the dipole moment of CN could change with a nano-droplet size. Furthermore, it was demonstrated that RPMI-1640 as a growth medium had failed to change NLO properties of CN encapsulated in nano-droplet. Accordingly, the encapsulated CN in nano-droplet in the form of a photosensitizer (PS) was suggested as a good candidate to examine PDT under in-vitro conditions.