Isolation, identification and biological evaluation of phytochemicals from : a medicinal plant endemic to Western Ghats of India.

The present investigation reports evaluation of phytochemical content, antimicrobial, antioxidant, antidiabetic and anticancer properties of the methanolic crude extracts prepared from leaves. Qualitative evaluation showed the presence of carbohydrates, reducing sugars, alkaloids, phenols, flavonoids, cardiac glycosides, steroids, phytosterols, terpenoids, diterpenoids and coumarins. Quantitative estimation for phenols, flavonoids and alkaloids indicated total phenolic content 11.95 mg GAE/g, total flavonoid content 20.34 mg QE/g and total alkaloid content 316.68 mg AE/g dry weight of the crude extract respectively. The methanolic extract demonstrated effective antimicrobial activity against , and The preparation also demonstrated good antioxidant and antidiabetic activities. Anticancer activity was evaluated against breast (MCF), oral (KB) and lung (A-549) cancer cell lines and the respective IC values were found to be 159.81 ± 7.54 µg/mL, 240.21 ± 2.57 µg/mL and 124.17 ± 2.10 µg/mL which are comparable to IC values of Paclitaxel.